Currently, the area of enzymatic manipulation of nucleic acids is expanding in several different directions. VIROINF will be timely by using available viral metagenome data to explore the great potential to search for new enzymes useful in biotechnology. New viral enzymes have a high chance of showing new and different properties that can open up new or improved applications and expand the market.
In particular thermophilic phages infecting bacteria and archaea (identified in WP 1.2) have been recognised as a novel source for nucleic acid modifying enzymes. In most DNA/RNA applications high temperature gives a clear advantage and therefore preference will be to search
for enzymes from thermophilic (or other extremophilic) metagenomics samples. ESR 7 and ESR 14 will tackle the area of treatment development against bacterial infections. This working package uses the output of WP 1.1, WP 1.2, and WP 1.4 to large-scale identification of phage sequences, functional annotation and in vitro validation of phages as a treatment against harmful bacteria.
VIROINF will interact in this working package closely with InfectoGnostics, emphasising close interactions to industrial partners for specific goals (in our case functions of selected proteins).
- ESR 7: Generation of datasets for phage-bacterial interactions in complex communities and mutational spectra analysis following exposure to selective pressure
- ESR 14: Computational methods for the analysis of metagenomic datasets to extract viral sequences within the context of commercial data-mining
Antibiotic Exposure Leads to Reduced Phage Susceptibility in Vancomycin Intermediate Staphylococcus aureus (VISA) Journal Article
In: Antimicrob Agents Chemother, 2022.
ITN -- VIROINF: Understanding (Harmful) Virus-Host Interactions by Linking Virology and Bioinformatics Journal Article
In: Viruses, vol. 13, no. 5, pp. 766, 2021.
Antibiotic Exposure Leads to Reduced Phage Susceptibility in Vancomycin Intermediate Staphylococcus aureus (VISA) Presentation
Poster at Viruses of Microbes (VoM) meeting 2022, 19.07.2022.
The database of Virus Orthologous Groups and its application in phage cocktail genomics Presentation
Poster at Viruses of Microbes (VoM) meeting 2022, 18.07.2022.
VOGDB - Virus Orthologous Groups database Presentation
Oral presentation at Quest for Orthologs 2021, 02.08.2021.